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Below we have summarized the published literature starting with those channels for which the most robust changes have been observed after gene targetingThe study of Asic gene knock-out mice has clearly implicated this ion channel family in mechanosensation, however, the apparent increase in certain mechanosensitive properties upon deletion of ASICs suggests that these channels function as regulators and modulators of mechanosensation, rather than transducers. Another possible explanation for the complex phenotypes observed in the knock-out studies would be functional redundancy of the remaining ASICs and/or converse rose gold compensatory effects.
Given that ASICs assemble as trimers (Carnally et al., 2008; Jasti et al., 2007) and that ASIC1-4 are expressed in DRG neurons, evidence suggests that different ASIC subunits assemble as heteromers in order to form proton-gated channels in mouse sensory neurons (Bensonet al., 2002). Therefore, deletion of one Asic gene could merely change the subunit composition of the remaining trimeric channels, the composition of which will of course depend on expression of ASIC subunits in each sensory neuron sub-type. As such, it might be expected that deletion of [img]http://www.impressdispatch.com/images/shoes/converse rose gold-513rcg.jpg[/img] multiple Asic genes would reveal more profound mechanosensory phenotypes.
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